human aspc1/luciferase stable cells  (GenTarget)

Journal: Cancers

Article Title: Externally Applied Electromagnetic Fields and Hyperthermia Irreversibly Damage Cancer Cells

doi: 10.3390/cancers15133413

Figure Lengend Snippet: Effect of EMFs and HT on cancer cell viability. ( A ) Effect of EMFs. Cancer cells were seeded and 24 h later exposed to EMFs (100–500 kHz × 1–5 h). Control values (0 kHz) were 1.14 ± 0.03 A2058, 1.13 ± 0.04 AsPC1, and 1.20 ± 0.05 MDA-MB-231 (×10 6 ) viable cells ( n = 5 in all cases). * p < 0.05 comparing all conditions versus controls (0 kHz) ( n = 5 t -test). ( B ) Effect of HT. Cancer cells were seeded and 24 h later exposed to HT (42–52 °C × 20–40 min). * p < 0.05 ** p < 0.01 comparing all conditions versus controls (37 °C) ++ p < 0.01 comparing 40 min versus 20 min ( n = 5 t test). ( C ) Effect of EMFS and HT. Cancer cells were seeded and 24 h later exposed to EMFs (100 kHz × 4 h) and HT (52 °C × 40 min from min 120 to min 160 of the 4 h period where cells were constantly exposed to the EMFs). The surviving cells were cultured for 24 additional hours without further exposure to EMFs and HT. A two-way analysis of variance (ANOVA) was used to make comparisons among the different groups after 4 h of treatment with EMFs + HT and 24 h after. Different letters indicate differences p < 0.05 ( n = 5).

Article Snippet: Human AsPC1/Luciferase Stable Cells were obtained from GenTarget Inc. (San Diego, CA, USA).

Techniques: Control, Cell Culture

Journal: Cancers

Article Title: Externally Applied Electromagnetic Fields and Hyperthermia Irreversibly Damage Cancer Cells

doi: 10.3390/cancers15133413

Figure Lengend Snippet: Effect of EMFs and HT on ROS generation and the molecular mechanisms of apoptosis.

Article Snippet: Human AsPC1/Luciferase Stable Cells were obtained from GenTarget Inc. (San Diego, CA, USA).

Techniques:

Journal: Cancers

Article Title: Externally Applied Electromagnetic Fields and Hyperthermia Irreversibly Damage Cancer Cells

doi: 10.3390/cancers15133413

Figure Lengend Snippet: Effect of EMFs + HIFU-induced HT, gemcitabine and/or PT on the growth of AsPC1 pancreas carcinoma. Cancer cells were inoculated subcutaneously on day 0, and mice were treated with EMFs and HIFU as described under Materials and Methods. ( A ) EMFs and HIFU were applied once per day per three consecutive days (Monday to Wednesday) for two consecutive weeks starting on day 14 after tumor inoculation. Gemcitabine (50 mg/kg) was administered twice on days 14 and 21. A one-way analysis of variance (ANOVA) was used to make comparisons among the different experimental groups at each time point. Different letters indicate statistical differences p < 0.05 ( n = 15 mice per experimental group). ( B ) A disodium salt of PT phosphate (Chromadex Inc. Los Angeles CA) (100 mg of PT/kg) was administered i.p. (one dose 30 min before starting each irradiation session with EMFs and HT). A one-way analysis of variance (ANOVA) was used to make comparisons among the different experimental groups. Different letters indicate statistical differences p < 0.05 ( n = 12 mice per experimental group). ( C ) Representative images of mice inoculated with AsPC1/Luciferase Stable Cells and treated with EMFs HT and gemcitabine (GEM) or EMFs HT gemcitabine and PT.

Article Snippet: Human AsPC1/Luciferase Stable Cells were obtained from GenTarget Inc. (San Diego, CA, USA).

Techniques: Irradiation, Luciferase